This stage of the tournament will feature 16 clubs — eight winners from the third preliminary round and eight teams from the Ukrainian Premier League (UPL) with the highest rankings from the previous season.

Vbet Ukrainian Cup 2024/2025. Round of 16 

October 29, 2024

Rukh (Lviv) vs. Karpaty (Lviv). Lviv, Arena Lviv Stadium. 18:00

October 30, 2024

Probiy (Horodenka) vs. Bukovyna (Chernivtsi). Horodenka, Probiy Arena. 12:00

UKSA (Tarasivka) vs. Oleksandriya (Oleksandriya). Kyiv, V. Bannikov Stadium. 13:00

Polissya (Zhytomyr) vs. Kryvbas (Kryvyi Rih). Zhytomyr, Central Stadium. 15:30

Vorskla (Poltava) vs. Dynamo (Kyiv). Poltava, O. Butovskyi Vorskla Stadium. 15:30

Shakhtar (Donetsk) vs. Zorya (Luhansk). Lviv, Arena Lviv Stadium. 18:00

Veres (Rivne) vs. Obolon (Kyiv). Rivne, Avangard Stadium. 18:00

Viktoriya (Sumy) vs. LNZ (Cherkasy). Cherkasy, Cherkasy Arena. 18:00

The remaining rounds are scheduled for 2025, and the final of the Ukrainian Cup for the 2024/2025 season will take place on May 14. The location will be determined by the UAF Executive Committee.